Waching daily Jan 29 2018

so the second method which i'll telling you about for ABO RH blood grouping is by tube method tube method is better than

the conventional slide into it because in the tube method we are doing both the forward

grouping a s well as the reverse grouping so both the grouping by forward

reverse are cross check so chances of HR minimum and that's why it is

called as a full standard for doing them ABO RH blood group so for this procedure

we required atleast 7 tubes which we have to label with the donor ID or with the

patient name the first tube we have to label as Anti-A

second tube Anti-B

Third tube Anti-AB

fourth tube Anti-D

and to do the reverse grouping we have another three tubes

with the first tube with the donor id or with the patient name and ID

We have to write A cell

B cell

and o cell

apart from this we need

Anti sera

Anti sera A

Anti sera B

Anti sera D

Anti sera AB

we also need

the cell suspension

which is should three to five percent of the cells suspension once we are separated the whole blood

into the cell and plasma

Plasma

of the donor or the patient

and commercially prepaid or in the blood bank prepaid

A cell

B cell

and

O cell

so to start with in the first tube will put two drops of Antisera-A

two drops

of Antisera B

two drops of Antisera

AB

and two drops

of Antisera D

after this

will be adding

one drop each of cell suspension

which is three to five percent prepare

one drop in Anti-A

one drop in Anti-B

one drop in Anti-AB

and one drop in Anti-D

to do the reverse typing

first we have to add

two drops

of plasma

in A cell

B cell

and

O cell

after that

commercially prepaid or in the blood bank prepaid

A cell

in the a cell one drop

B cell

one drop in B cell

and

O cell

one drop

after this with gentle shaking we will keep them in the centrifuge

one minute

at 1000rpm

other after gentrification for one minute

at 1000rpm

we will see

that

there is a button shape O+ agglutination

in

Antisera A tube

in the second tube of Antisera B again there is a button shape O+ agglutination

in the third tube again Antisera AB

their is a O+ agglutination

and

in the fourth tube also

of Anti-D there is

a button shape O+ agglutination of Anti-D

so by over the forward typing it is Ab positive

now we have to do the cross check with the reverse type

in the a cell

there is no agglutination

in the B cell

there is no agglutination

and

in the O cell also

there is no agglutination

so

as

it is giving agglutination at O+ reaction in Anti-A Anti-B Anti-AB and Anti-D and in reverse

it is not giving reaction in any of the A cell B cell O cell so again the blood group of the patient

is AB positive